De-coding and re-coding RNA recognition by PUF and PPR repeat proteins
نویسندگان
چکیده
منابع مشابه
A universal code for RNA recognition by PUF proteins.
The design of proteins that can bind any RNA sequence of interest has many potential biological and medical applications. Here we have expanded the recognition of Pumilio and FBF homology protein (PUF) repeats beyond adenine, guanine and uracil and evolved them to specifically bind cytosine. These repeat sequences can be used to create PUF domains capable of selectively binding RNA targets of d...
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Protein mimicry of DNA is a recently discovered direct mechanism of regulation of DNAdependent enzyme activity by means of proteins that mimic DNA structure and interact with a target enzyme and completely inhibit (or modulate) its activity. DNA-mimicking inhibitor proteins bind directly to the enzyme and thus blocks or alters the activity of the latter. Protein mimicry of DNA was first describ...
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Summary: SOX2 overlapping transcript (SOX2OT) is a long non-coding RNA associated with cancer pathogenesis. It contributes to a variety of cellular functions and recent evidence propounds its association with autophagy process. It has been showed that SOX2OT can regulate the expression of different autophagy associated factors in human cells with different mechanisms, however more remains to ...
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Pentatricopeptide repeat (PPR) proteins are helical-repeat proteins that offer a promising scaffold for the engineering of proteins to bind specified RNAs. PPR tracts bind RNA in a modular 1-repeat, 1-nucleotide fashion. An amino acid code specifying the bound nucleotide has been elucidated. However, this code does not fully explain the sequence specificity of native PPR proteins. Furthermore, ...
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ژورنال
عنوان ژورنال: Current Opinion in Structural Biology
سال: 2016
ISSN: 0959-440X
DOI: 10.1016/j.sbi.2016.01.010